Johns Hopkins University
Research Technologist
Johns Hopkins University, Baltimore, Maryland, United States, 21276
The Department of Neurology is seeking a
Research Technologist
for amolecular virology (HIV-1, biosafety level 2) laboratory. The Research Technologist will utilize a solid foundation in basic laboratory techniques, molecular biology and primary cell culture requiring a high level of rigor and reproducibility. The Research Technologist will exercise independent judgment while participating in the planning of experiments and interpreting results. The projects require care in data collection, preparation of reports and interfacing with sponsors. The work requires occasional irregular work hours consistent with research needs related to in vivo studies with mice.
Specific Duties & ResponsibilitiesMouse breeding and husbandry.Handling and performing blood draw and injections on mice infected with viral pathogens.Isolation of peripheral blood lymphocytes and monocytes from human blood.Perform in vitro infection studies with HIV-1.Perform general molecular biology techniques such as cloning, qPCR, and Western blotting.Perform specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.Provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
Additional Knowledge, Skills & AbilitiesWilling to learn Isolation of PBMCs and culture of primary human cells; sterile technique.Willing to learn and implement new technologies.
Ability for self-management, integrity and professionalism.
Work effectively as a team-member and exhibit excellent interpersonal, organizational, and self-management skills.
Minimum QualificationsBachelor's Degree in Biology, Chemistry or related field.Willing to work under Biohazard Level 2 Safety conditions with HIV-1.Flexibility to occasionally work irregular hours consistent with research needs.
Preferred QualificationsMaster's Degree in Biological Sciences preferred.Some related post-undergraduate work experience.
Experience and or interest in learning how to conduct single-cell genomics and computational analyses.
Classified Title: Research TechnologistRole/Level/Range: ACRO40/E/02/CCStarting Salary Range: $15.70 - $26.25 HRLY (Commensurate with experience)Employee group: Full TimeSchedule: M-F 8:30 am - 5:00 pmExempt Status:Non-ExemptLocation:School of Medicine CampusDepartment name: SOM Neuro NeuroimmunologyPersonnel area: School of Medicine
Research Technologist
for amolecular virology (HIV-1, biosafety level 2) laboratory. The Research Technologist will utilize a solid foundation in basic laboratory techniques, molecular biology and primary cell culture requiring a high level of rigor and reproducibility. The Research Technologist will exercise independent judgment while participating in the planning of experiments and interpreting results. The projects require care in data collection, preparation of reports and interfacing with sponsors. The work requires occasional irregular work hours consistent with research needs related to in vivo studies with mice.
Specific Duties & ResponsibilitiesMouse breeding and husbandry.Handling and performing blood draw and injections on mice infected with viral pathogens.Isolation of peripheral blood lymphocytes and monocytes from human blood.Perform in vitro infection studies with HIV-1.Perform general molecular biology techniques such as cloning, qPCR, and Western blotting.Perform specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.Provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.
Additional Knowledge, Skills & AbilitiesWilling to learn Isolation of PBMCs and culture of primary human cells; sterile technique.Willing to learn and implement new technologies.
Ability for self-management, integrity and professionalism.
Work effectively as a team-member and exhibit excellent interpersonal, organizational, and self-management skills.
Minimum QualificationsBachelor's Degree in Biology, Chemistry or related field.Willing to work under Biohazard Level 2 Safety conditions with HIV-1.Flexibility to occasionally work irregular hours consistent with research needs.
Preferred QualificationsMaster's Degree in Biological Sciences preferred.Some related post-undergraduate work experience.
Experience and or interest in learning how to conduct single-cell genomics and computational analyses.
Classified Title: Research TechnologistRole/Level/Range: ACRO40/E/02/CCStarting Salary Range: $15.70 - $26.25 HRLY (Commensurate with experience)Employee group: Full TimeSchedule: M-F 8:30 am - 5:00 pmExempt Status:Non-ExemptLocation:School of Medicine CampusDepartment name: SOM Neuro NeuroimmunologyPersonnel area: School of Medicine