University of Miami
Associate Scientist - Proteomics
University of Miami, Miami, Florida, us, 33222
Associate Scientist - ProteomicsLocation: Miami, FL
Time Type: Full time
Posted on: 12 Days Ago
Job Requisition ID: R100073881
Department Specific Functions
The Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine is looking for an Associate Scientist to join the new Sylvester Proteomic Shared Resource (PSR), to apply state of the art LC-MS based methods and techniques for quantitative proteomics.
As an Associate Scientist in the PSR, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. The individual will:
Be the primary person responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
Manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels).
Implement both bottom-up & top-down proteomic approaches.
Be responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g., SILAC, TMT).
Be responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
Conduct single-cell proteomics analysis.
Perform intact protein analysis & MW determination.
Use proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
Be responsible for operation, maintenance, and troubleshooting of an Orbitrap Ascend Tribrid Mass Spectrometer and other laboratory equipment as needed.
Assess experimental results and troubleshoot methods and instruments to optimize research protocols.
Collaborate with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues.
Education:
Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field).
Experience:
Minimum 5 years of relevant experience, including 2 years of experience in proteomics mass spectrometry applications.
Knowledge, Skills and Attitudes:
Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment.
Strong interest in handling, processing, and analyzing high-throughput datasets.
Motivation to constantly develop skills and expertise.
Ability to work both independently and as a team member.
Excellent analytical, communication, problem solving, and organizational skills.
Established track record of publications and demonstrated ability to complete projects.
Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.
Preference will be given:
Expertise in one or more of the following technical areas: quantitative proteomics, single cell proteomic analysis, global proteome profiling.
Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems.
The University of Miami offers competitive salaries and a comprehensive benefits package including medical, dental, tuition remission and more.
The University of Miami is an Equal Opportunity Employer - Females/Minorities/Protected Veterans/Individuals with Disabilities are encouraged to apply.
Job Status:
Full time
Employee Type:
Staff
Pay Grade:
A11
#J-18808-Ljbffr
Time Type: Full time
Posted on: 12 Days Ago
Job Requisition ID: R100073881
Department Specific Functions
The Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine is looking for an Associate Scientist to join the new Sylvester Proteomic Shared Resource (PSR), to apply state of the art LC-MS based methods and techniques for quantitative proteomics.
As an Associate Scientist in the PSR, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. The individual will:
Be the primary person responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
Manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels).
Implement both bottom-up & top-down proteomic approaches.
Be responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g., SILAC, TMT).
Be responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
Conduct single-cell proteomics analysis.
Perform intact protein analysis & MW determination.
Use proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
Be responsible for operation, maintenance, and troubleshooting of an Orbitrap Ascend Tribrid Mass Spectrometer and other laboratory equipment as needed.
Assess experimental results and troubleshoot methods and instruments to optimize research protocols.
Collaborate with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues.
Education:
Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field).
Experience:
Minimum 5 years of relevant experience, including 2 years of experience in proteomics mass spectrometry applications.
Knowledge, Skills and Attitudes:
Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment.
Strong interest in handling, processing, and analyzing high-throughput datasets.
Motivation to constantly develop skills and expertise.
Ability to work both independently and as a team member.
Excellent analytical, communication, problem solving, and organizational skills.
Established track record of publications and demonstrated ability to complete projects.
Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.
Preference will be given:
Expertise in one or more of the following technical areas: quantitative proteomics, single cell proteomic analysis, global proteome profiling.
Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems.
The University of Miami offers competitive salaries and a comprehensive benefits package including medical, dental, tuition remission and more.
The University of Miami is an Equal Opportunity Employer - Females/Minorities/Protected Veterans/Individuals with Disabilities are encouraged to apply.
Job Status:
Full time
Employee Type:
Staff
Pay Grade:
A11
#J-18808-Ljbffr