Research Assistant

Job Description - Research Assistant (3309612)A Research Assistant position is available in the Oyoshi laboratory at Massachusetts General Hospital for Children to study the immune mechanisms of food allergy. The position will acquire a variety of skills that are required for in vitro and in vivo analysis of mouse models of allergic diseases, which will be instrumental for the development of the staff’s career.Minimum Qualifications:Excellent writing and communication skills in EnglishPrevious experiences with handling and managing experimental animal coloniesBasic biology techniques, including sterile cell culture, gel electrophoresis, PCR, DNA/RNA extractionCandidates who are familiar with immunological techniques are desirable:Primary cell isolationELISAQuantitative PCRMulti color flow cytometryAnimal models of human disease, preferably allergic inflammationThe priority will be given to applicants with an excellent background in Immunology. If applicable, publication(s) in international journals in the Immunology area would be a plus. Please send a brief statement of your research experience and career goals (limit to one half page), curriculum vitae with bibliography, and names and contact information of three references in a single PDF file to Dr. Michiko Oyoshi at moyoshi@mgh.harvard.edu. Incomplete applications will not be reviewed.Principal Duties and Responsibilities:Animal Colony:Manages experimental animal colonies: daily maintenance, breeding, crossing, and genotypingMaintains weekly animal colony records and databasesScience:Plans, setups, and performs routine in vivo/in vitro experimentsCollects and organizes experimental samples and dataMaintains experimental records and databasesPrepares reagents and solutionsAssists lab members for conducting experimentsTroubleshoots assays, methods, equipment to obtain high quality dataUses software programs to generate graphs and reports, including but not limited to, Word, Excel, Prism, PowerPoint, AdobeConducts literature searchesManagement:Orders and maintains inventory of laboratory supplies/consumablesManages institutional protocols including IACUC, IBC, and IRBEnsures daily operations of laboratory, including maintaining equipment, protocols, research inventories, daily budgetPerforms administrative support duties as requiredSkills/Abilities/Competencies Required:Careful attention to detailsExcellent organizational skillsAbility to follow directionsAbility to work independentlyFlexibility and willingness to complete tasksKnowledge of research protocolsEducation:Bachelor's degree required.Experience:Basic biology techniques; requiredWorking Conditions:Works under general supervision in laboratory setting.Supervisor regularly reviews progress of work.Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives, and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.

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