Brigham and Women’s Hospital
Research Lab Technician II BWH Rheumatology
Brigham and Women’s Hospital, Boston, Massachusetts, us, 02298
Job Description - Research Lab Technician II BWH Rheumatology (3312680)This position is in the laboratory of Ellen Gravallese, MD, Chief of the Division of Rheumatology, Inflammation, and Immunity. Our group is studying inflammation and osteoimmunology in the context of autoimmune diseases such as rheumatoid arthritis and spondylarthritis. In addition, we study innate immune mechanisms of autoimmune and autoinflammatory diseases. We utilize animal models of disease, human tissue samples, and in vitro experiments to study the pathways by which inflammation is mediated and also by which bone homeostasis is disrupted in disease states.We are looking for a motivated and organized individual with hands-on experience working in the laboratory. The person filling this position will work closely with senior members of the lab, supporting them in various aspects of their ongoing research projects. In addition, it would be ideal if this candidate could also assist in our animal facility. The ideal candidate will have experience performing ELISAs, Westerns, primary cell culture, qPCR, and histology. Experience in animal husbandry, genotyping (PCR), dissection and tissue harvesting, and organization of the animal database is also desirable, but not required. Finally, experience with analysis of bulk and single cell RNA sequencing data and the use of R would be an advantage.Principal Duties and Responsibilities
Bench workCarries out the following:RT-PCR/qPCRELISAPrimary cell cultureRNA extractionWesternsMicroscopyFACs analysisSample preparation for RNA sequencingResponsible for the continual maintenance of the laboratory.Contributes to scientific literature.Attends regular meetings of the Principal Investigators to discuss improvements on the general operation of the laboratory and acts upon decisions made.Has working knowledge of databases/spreadsheets software (Microsoft Office).Responsible for maintenance of mouse colony, including weaning, ear tagging, genotyping by PCR, setting up breeding and record keeping.Keeping an up-to-date laboratory notebook.Responsible for maintaining lab space, equipment, and supplies.Ability to learn new laboratory techniques and to teach others as needed.Ability to communicate well, both verbally and in writing.Good organizational skills.Ability to work closely with the research team.Self-motivated and capable of troubleshooting and creative problem solving.Qualifications
Bachelor’s degree or equivalent required (suitable field preferred).Minimum of 1 year experience working in a laboratory.Relevant biology coursework is preferred.Demonstrated organizational and interpersonal skills as required to work closely with a research team.Demonstrated competence with databases/spreadsheets and software packages.BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives, and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.Primary Location
MA-Boston-BWH Longwood Medical AreaWork Locations
BWH Longwood Medical Area75 Francis StreetJob
Organization
Brigham & Women's Hospital (BWH)Schedule
Full-timeStandard Hours 40Shift
Day JobEmployee Status
Regular
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Bench workCarries out the following:RT-PCR/qPCRELISAPrimary cell cultureRNA extractionWesternsMicroscopyFACs analysisSample preparation for RNA sequencingResponsible for the continual maintenance of the laboratory.Contributes to scientific literature.Attends regular meetings of the Principal Investigators to discuss improvements on the general operation of the laboratory and acts upon decisions made.Has working knowledge of databases/spreadsheets software (Microsoft Office).Responsible for maintenance of mouse colony, including weaning, ear tagging, genotyping by PCR, setting up breeding and record keeping.Keeping an up-to-date laboratory notebook.Responsible for maintaining lab space, equipment, and supplies.Ability to learn new laboratory techniques and to teach others as needed.Ability to communicate well, both verbally and in writing.Good organizational skills.Ability to work closely with the research team.Self-motivated and capable of troubleshooting and creative problem solving.Qualifications
Bachelor’s degree or equivalent required (suitable field preferred).Minimum of 1 year experience working in a laboratory.Relevant biology coursework is preferred.Demonstrated organizational and interpersonal skills as required to work closely with a research team.Demonstrated competence with databases/spreadsheets and software packages.BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives, and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.Primary Location
MA-Boston-BWH Longwood Medical AreaWork Locations
BWH Longwood Medical Area75 Francis StreetJob
Organization
Brigham & Women's Hospital (BWH)Schedule
Full-timeStandard Hours 40Shift
Day JobEmployee Status
Regular
#J-18808-Ljbffr