Laboratory Research Technician

Laboratory Research Technician This position will provide technical support to an NIH funded laboratory in the Department of Medicine's Renal-Electrolyte Division. This lab employs molecular biology, cellular biology, and physiology, to study cell stress and kidney electrolyte regulation. The candidate will work closely with the supervisor to assist in performing experiments, analyzing data, and maintaining the lab's inventory, mouse colonies, cell lines, and safety records. S/he must be open to the possibility of working with animals rodents. A bachelor's degree in biology, chemistry, or other basic science is required, and prior wet lab research experience is preferred. The successful candidate will be a highly organized individual with good communication skills, who has the motivation and personality to work productively with others in a collegial atmosphere. Job Summary Performs laboratory testing and some analysis on multiple types of specimen samples. Operates and properly sterilizes equipment and maintains inventory and assists with ordering. Essential Functions Key Job Duties Molecular cloning, western blotting, cell culture, gene editing CRISPR/Cas9; with guidance from the PI, mouse genotyping, fixed and live cell confocal microscopy, and work with genetically modified mice tissue, blood and urine collection/analysis Operates and maintains applicable laboratory equipment for job functions. Keeps detailed, accurate, and timely data and log books. Organizes and documents results. Sterilizes, maintains, and cleans lab equipment; prepares work areas; and maintains cleanliness Provide technical support for other research scientists in the lab faculty, postdocs, graduate students Physical Effort Requires some bending, stooping, and stretching. Walking around laboratory benches and various common laboratory rooms within the Division. The candidate will use stairs often, and may have to make multiple trips between common equipment rooms of the 8th and 9th floors of Scaife hall on some days. Requires normal range of hearing and eyesight to record, prepare, and communicate laboratory methods and results. Requires lifting up to 20lbs. The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all. The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets. Assignment Category: Full-time regular Job Classification: Staff.Laboratory Research Technician I Job Family: Research Job Sub Family: Laboratory Campus: Pittsburgh Minimum Education Level Required: Bachelors Degree Minimum Years of Experience Required: No experience required Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: Monday - Friday, 8.00a - 4.30p designated timeframe flexible Work Arrangement: Monday - Friday, 8.00a - 4.30p designated timeframe flexible Hiring Range: TBD Based Upon Qualifications Relocation_Offered: No Visa Sponsorship Provided: No Background Check: For position finalists, employment with the University will require successful completion of a background check Child Protection Clearances: Not Applicable Required Documents: Resume Optional Documents: Cover Letter PI254958264