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University of Washington

RESEARCH SCIENTIST/ENGINEER 3

University of Washington, Seattle, Washington, us, 98127


If you are using a screen reader and experience any difficulty accessing our web pages, please call 206-543-2544 or email UWHires and we will be happy to assist you. jump to content Department: DEPARTMENT OF LABORATORY MEDICINE AND PATHOLOGY Job Location: Seattle Campus Job Location Detail: Health Sciences Building Posting Date: 12/09/2024 Closing Info: Open Until Filled Other Compensation: Union Position: Yes Shift: First Shift Benefits: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills, and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits, and natural beauty.

The Department of Laboratory Medicine and Pathology has an outstanding opportunity for a Research Scientist/Engineer 3 (RSE UAW) to join the Bitto Lab.

The Bitto Lab investigates the biological effects of longevity interventions on mitochondrial function and nutrient metabolism. By utilizing both in vitro and rodent models, we seek to uncover the cellular and systemic biological pathways that contribute to life extension and healthy aging.

DUTIES AND RESPONSIBILITIES

General Lab Functions (20%): Laboratory Management: Oversee daily lab operations, ensuring compliance with EH&S regulations, including BSL-1/BSL-2 inspections, BUA approval, and maintaining all required documentation. Monitor and manage laboratory supplies, ensuring that junior staff is keeping an adequate inventory and ensuring a timely restocking.

Training and Mentorship: Provide training to new lab personnel on experimental protocols, laboratory safety, and data management. Mentor undergraduate and graduate students in research techniques, hypothesis generation, and experimental design.

Experimental and Research Functions (80%):

Cell Culture and Transgene Expression: Manage tissue culture operations, including cell line propagation, transgene expression, and subsequent characterization. Utilize recombinant DNA technology and viral vectors for genetic manipulation.

Organellar Function Analysis: Conduct assays to measure mitochondrial and organellar function and dynamics, with a focus on understanding the molecular mechanisms underlying aging and longevity interventions.

Biomolecular Analysis: Perform extraction and analysis of nucleic acids and proteins to evaluate cellular expression and turnover. Conduct protein assays, Western blots, RT-PCR, and other molecular techniques.

Experimental Design and Execution: Contribute to hypothesis generation, experimental planning, and execution. Design experiments to test specific research questions regarding mitochondrial function and aging pathways. Collaborate with post-doctoral researchers to advance key projects.

Collaboration and Communication: Work closely with research staff and faculty, contributing to the development of research objectives and strategic goals. Participate in the preparation of manuscripts, including creating figures, analyzing data, and contributing to the writing process.

MINIMUM REQUIREMENTS

Bachelor’s degree in Biochemistry, Cellular Biology, Genetics, or related field AND four (4) years of relevant professional laboratory experience working with mitochondrial function and nutrient metabolism, both in vitro and rodent models.

Proficiency with BSL2+ laboratory procedures required.

Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

ADDITIONAL REQUIREMENTS Specific knowledge, skills and abilities required to perform the job satisfactorily include:

Cell Culture & Recombinant DNA Technology: Proven ability to work with mammalian cell cultures, including transfection, viral vector use, and managing cell line propagation.

Flow Cytometry: Experience with flow cytometry, including sample preparation, instrument calibration, and data interpretation.

Biochemical and Molecular Assays: Expertise in protein and nucleic acid-based assays such as Western blotting, RT-PCR, and other related techniques to assess cellular processes.

Laboratory Safety & Compliance: Comprehensive knowledge of laboratory safety protocols, especially with BSL-2 and BSL-2+ work, and experience managing compliance and safety training.

DESIRED QUALIFICATIONS

Advanced Degree: Doctoral degree (PhD) in Biochemistry, Cellular Biology, Genetics, or a related medical/scientific field.

Mitochondrial Function Measurements: Advanced experience with measuring mitochondrial bioenergetics, function, and morphology.

Research Management: Experience in managing or coordinating research projects, contributing to grants, and working collaboratively with faculty and external stakeholders.

Scientific Communication: Strong communication skills, including the ability to present findings at conferences, prepare publications, and collaborate across teams.

Application Process:

The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming. To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu . Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law .

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