University of California
Staff Research Associate I - Pediatrics
University of California, Los Angeles, California, United States, 90079
Description
Under supervision of the Principal Investigator, the Lab Manager, and the Senior Postdoctoral Scholars, the Staff Research Associate 1 (SRA 1) will conduct experiments investigating the design of cellular therapeutic manufacturing platforms that leverage nanotechnology-enabled methods for biomolecular nanoparticle-based packaging and intracellular delivery. The SRA 1 will be empowered to lead efforts aimed at tailoring lipid nanoparticle structures with targeting functionalities using click chemistry approaches and conduct their work independently. Responsibilities include but are not limited to: nanoparticle synthesis and characterization, tissue culture, molecular biology analyses such as flow cytometry, Western blot, gel electrophoresis, PCR, immunohistochemistry, confocal microscopy, electron microscopy, etc. In addition, the SRA 1 will assist laboratory personnel with experiments as needed, provide for maintenance of equipment related to nanoparticle preparation and microfluidic device prototyping.Please note that this is a full-time 18 month limited positionand may convert to career.Salary Range: $26.52 - $31.63/HourlyQualifications
All Required:
The ability to culture andmaintain human primary cells and cell lines.Experience in nanoparticlesynthesis and/or working with microfluidic devices.Organize and researchproject-related records, data, and experimental protocolsExperience withscanning/transmission electron microscopy.Experience withimmunostaining, flow cytometry, and confocal imaging.Perform RNA extraction,MaxiPrep, and qPCR.Critically evaluate therelevant literature on similar techniques to troubleshoot and adapt theseprotocols.Experience working withother lab scientists to troubleshoot protocols and procedures.
Under supervision of the Principal Investigator, the Lab Manager, and the Senior Postdoctoral Scholars, the Staff Research Associate 1 (SRA 1) will conduct experiments investigating the design of cellular therapeutic manufacturing platforms that leverage nanotechnology-enabled methods for biomolecular nanoparticle-based packaging and intracellular delivery. The SRA 1 will be empowered to lead efforts aimed at tailoring lipid nanoparticle structures with targeting functionalities using click chemistry approaches and conduct their work independently. Responsibilities include but are not limited to: nanoparticle synthesis and characterization, tissue culture, molecular biology analyses such as flow cytometry, Western blot, gel electrophoresis, PCR, immunohistochemistry, confocal microscopy, electron microscopy, etc. In addition, the SRA 1 will assist laboratory personnel with experiments as needed, provide for maintenance of equipment related to nanoparticle preparation and microfluidic device prototyping.Please note that this is a full-time 18 month limited positionand may convert to career.Salary Range: $26.52 - $31.63/HourlyQualifications
All Required:
The ability to culture andmaintain human primary cells and cell lines.Experience in nanoparticlesynthesis and/or working with microfluidic devices.Organize and researchproject-related records, data, and experimental protocolsExperience withscanning/transmission electron microscopy.Experience withimmunostaining, flow cytometry, and confocal imaging.Perform RNA extraction,MaxiPrep, and qPCR.Critically evaluate therelevant literature on similar techniques to troubleshoot and adapt theseprotocols.Experience working withother lab scientists to troubleshoot protocols and procedures.