Pfizer, S.A. de C.V
Scientist (non-PhD), Post-CAN Research, Cancer Cell Programs
Pfizer, S.A. de C.V, California, Missouri, United States, 65018
Scientist (non-PhD), Post-CAN Research, Cancer Cell Programs
We’re in relentless pursuit of breakthroughs that change patients’ lives. We innovate every day to make the world a healthier place.Pfizer’s Oncology Research Unit (ORU) in La Jolla, California, is seeking a self-motivated and collaborative Scientist (non-PhD) to join the Post-CAN research group focused on translating basic discoveries in the field of cancer cell biology into novel therapeutics.The Scientist will participate in conducting preclinical research on our clinical and pre-clinical drug programs with a focus on oncogenic signaling and cell cycle assets. The Scientist will work as a member of the Cancer Cell program team to support translational research efforts utilizing pharmacological and genetic approaches to investigate target mechanism of action, intrinsic and acquired resistance, patient stratification strategies and drug combinations. The candidate will serve as a technical and scientific expert in the lab and will work independently and in collaboration with other team members to design, execute and analyze/interpret experiments. The successful candidate will demonstrate a proven track record of scientific productivity and high-level of technical competence in a wide range of in vitro pharmacology and cellular/molecular biology techniques.ROLE RESPONSIBILITIESWork independently and as part of teams to design, execute, analyze/interpret, and refine experiments ranging from in vitro pharmacology, biomarker assay development, functional cellular assays, molecular biology and in vivo tissue sample analysis.Apply genetic and pharmacologic approaches including CRISPR, RNAi, antibodies, small molecules etc. to modulate targets in vitro and assess functional impact on cancer initiation and progression.Execute experiments to advance mechanism of action studies, evaluate patient responder hypotheses, elucidate mechanisms of resistance, and assess rational drug combinations.Conduct cell-based assays across various technology platforms - qPCR, immunoprecipitation, FACS, immunofluorescence, western blotting, ELISA, high content screening, etc.Develop and apply complex in vitro assays for target validation and secondary pharmacology: spheroid models, colony forming assays, 3D co-culture systems, organoid systems.Collaborate with in vivo pharmacology group to support in vivo efficacy testing and assess target modulation in tumor tissues using supporting assays (ELISA, western blot, gene expression analysis of tissue lysates).Proactively seek out, embrace, and validate new technology/methods that can be incorporated into individual project(s) as well as the overall program.Use electronic data capture/analysis tools such as Excel, GraphPad, Biobook, Spotfire and PowerPoint for experimental documentation and data analysis/presentations.Present research results and methodologies at weekly meetings with immediate supervisor, research project meetings, PCR group meetings and other forums.Contribute to building a culture that embraces scientific excellence, urgency, partnerships with key stakeholders, continuous learning and improvement, increasing technical skill base and cancer biology expertise; take a proactive role in personal growth and scientific development.QUALIFICATIONSBasic QualificationsBachelor’s degree with 6+ or Master’s degree with 4+ years of experience in Molecular or Cellular Biology, Biochemistry, Genetics/Genomics, Pharmacology, or related field in an academic or pharmaceutical/biotech environment.A proven high-level of technical competence and extensive experience in a wide range of in vitro pharmacology and cellular/molecular biology techniques.Preference for candidates with a strong track record in cancer drug discovery and/or translational research focusing on cell cycle or/and cell signaling.Strong ability to multi-task, work efficiently and productively in a fast-paced, highly collaborative team environment.Strong attention to detail and organizational skills; excellent communication skills.Experience in use of CRISPR-based techniques for gene knockout, gene editing and working knowledge of pooled CRISPR-based screening approaches.
Work Location Assignment: On PremiseThe annual base salary for this position ranges from $74,900.00 to $124,800.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage.Relocation assistance may be available based on business needs and/or eligibility.
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We’re in relentless pursuit of breakthroughs that change patients’ lives. We innovate every day to make the world a healthier place.Pfizer’s Oncology Research Unit (ORU) in La Jolla, California, is seeking a self-motivated and collaborative Scientist (non-PhD) to join the Post-CAN research group focused on translating basic discoveries in the field of cancer cell biology into novel therapeutics.The Scientist will participate in conducting preclinical research on our clinical and pre-clinical drug programs with a focus on oncogenic signaling and cell cycle assets. The Scientist will work as a member of the Cancer Cell program team to support translational research efforts utilizing pharmacological and genetic approaches to investigate target mechanism of action, intrinsic and acquired resistance, patient stratification strategies and drug combinations. The candidate will serve as a technical and scientific expert in the lab and will work independently and in collaboration with other team members to design, execute and analyze/interpret experiments. The successful candidate will demonstrate a proven track record of scientific productivity and high-level of technical competence in a wide range of in vitro pharmacology and cellular/molecular biology techniques.ROLE RESPONSIBILITIESWork independently and as part of teams to design, execute, analyze/interpret, and refine experiments ranging from in vitro pharmacology, biomarker assay development, functional cellular assays, molecular biology and in vivo tissue sample analysis.Apply genetic and pharmacologic approaches including CRISPR, RNAi, antibodies, small molecules etc. to modulate targets in vitro and assess functional impact on cancer initiation and progression.Execute experiments to advance mechanism of action studies, evaluate patient responder hypotheses, elucidate mechanisms of resistance, and assess rational drug combinations.Conduct cell-based assays across various technology platforms - qPCR, immunoprecipitation, FACS, immunofluorescence, western blotting, ELISA, high content screening, etc.Develop and apply complex in vitro assays for target validation and secondary pharmacology: spheroid models, colony forming assays, 3D co-culture systems, organoid systems.Collaborate with in vivo pharmacology group to support in vivo efficacy testing and assess target modulation in tumor tissues using supporting assays (ELISA, western blot, gene expression analysis of tissue lysates).Proactively seek out, embrace, and validate new technology/methods that can be incorporated into individual project(s) as well as the overall program.Use electronic data capture/analysis tools such as Excel, GraphPad, Biobook, Spotfire and PowerPoint for experimental documentation and data analysis/presentations.Present research results and methodologies at weekly meetings with immediate supervisor, research project meetings, PCR group meetings and other forums.Contribute to building a culture that embraces scientific excellence, urgency, partnerships with key stakeholders, continuous learning and improvement, increasing technical skill base and cancer biology expertise; take a proactive role in personal growth and scientific development.QUALIFICATIONSBasic QualificationsBachelor’s degree with 6+ or Master’s degree with 4+ years of experience in Molecular or Cellular Biology, Biochemistry, Genetics/Genomics, Pharmacology, or related field in an academic or pharmaceutical/biotech environment.A proven high-level of technical competence and extensive experience in a wide range of in vitro pharmacology and cellular/molecular biology techniques.Preference for candidates with a strong track record in cancer drug discovery and/or translational research focusing on cell cycle or/and cell signaling.Strong ability to multi-task, work efficiently and productively in a fast-paced, highly collaborative team environment.Strong attention to detail and organizational skills; excellent communication skills.Experience in use of CRISPR-based techniques for gene knockout, gene editing and working knowledge of pooled CRISPR-based screening approaches.
Work Location Assignment: On PremiseThe annual base salary for this position ranges from $74,900.00 to $124,800.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage.Relocation assistance may be available based on business needs and/or eligibility.
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