University of California , San Francisco
Junior Specialist / Assistant Specialist - Virology & Proteomics (Krogan lab, UC
University of California , San Francisco, San Francisco, California, United States, 94199
Application Window
Open date:
February 27, 2024
Most recent review date:
Thursday, Oct 10, 2024 at 11:59pm (Pacific Time)
Applications received after this date will be reviewed by the search committee if the position has not yet been filled.
Final date:
Wednesday, Aug 27, 2025 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.
Position description
Junior Specialist / Assistant Specialist - Virology & Proteomics (Krogan lab, UCSF)
Your work will focus on mapping protein interactions and pathways involved in Human Immunodeficiency Virus (HIV) infection and latency. This role is an excellent opportunity to learn several valuable, cutting-edge techniques in molecular biology, virology, CRISPR gene-editing as well as proteomics while working with a dynamic and interdisciplinary research team. You will learn and apply different cellular, molecular, and biochemical assays to validate these findings. The candidate will also have the opportunity to develop and implement new protocols for gene-editing and mass spectrometry-based proteomics experiments.
Working as part of the infectious biology group in the Krogan lab, you will express affinity-tagged proteins of interest, perform affinity purifications, and proximity labeling of proteins and their interactors for mass spectrometry analysis to study protein-protein interactions. Additional major responsibilities will include the preparation, sequencing, and analysis of next-generation sequencing libraries to support CRISPR screening experiments. Further responsibilities will consist of isolation and culture of primary human immune cells, as well as functional analysis of engineered cells via Sanger sequencing and flow cytometry. The projects will also involve the purification of infectious HIV and infection of T cells with HIV in a biosafety level-3 facility. Strong organizational and communication skills are required.
The Krogan Lab is a highly dynamic and collaborative laboratory focusing on molecular networks underpinning infectious disease, carcinogenesis, and neurological disorders. Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently. Candidate's CV and/or cover letter must state basic qualifications (or if pending) upon submission. Candidates must meet all qualifications by the time of hire.
Required Qualifications:
Junior Specialist- a bachelor's degree (or equivalent degree) or at least four years of research experience.
Assistant Specialist- a master's degree or a bachelor's degree with three or more years of research experience.
Experience in the essential molecular biology techniques of molecular cloning, PCR, and
western blotting.
Experience in cell/tissue culture.
Able to work independently and meet deadlines; recognize and resolve problems while
maintaining a high level of organization.
Effective verbal and written communication skills to interface with the team to review and
analyze the results.
Willingness to work with infectious HIV virus in a BSL-3 facility
Willingness and ability to learn new methods and skills for changing research priorities
Excellent organizational and interpersonal communication skills (verbal and written)
Preferred Qualifications:
Experience transfection or viral transduction.
Experience with affinity purifications of proteins.
Experience with CRISPR/gene editing techniques
Experience with immunoprecipitation.
Experience in preparation of samples for mass spectrometric analysis.
Experience in fluorescence microscopy.
Experience with mass spectrometry data.
Experience with managing large datasets and/or databases.
Experience with statistical data analysis.
Please apply online at https://aprecruit.ucsf.edu/JPF04948, with a CV, cover letter, and three references.
See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$65,900.
Application Requirements
Document requirements
Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).
Cover Letter
Misc / Additional (Optional)
Reference requirements
3 required (contact information only)
About UC San Francisco
As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Job locationSan Francisco, CA
Open date:
February 27, 2024
Most recent review date:
Thursday, Oct 10, 2024 at 11:59pm (Pacific Time)
Applications received after this date will be reviewed by the search committee if the position has not yet been filled.
Final date:
Wednesday, Aug 27, 2025 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.
Position description
Junior Specialist / Assistant Specialist - Virology & Proteomics (Krogan lab, UCSF)
Your work will focus on mapping protein interactions and pathways involved in Human Immunodeficiency Virus (HIV) infection and latency. This role is an excellent opportunity to learn several valuable, cutting-edge techniques in molecular biology, virology, CRISPR gene-editing as well as proteomics while working with a dynamic and interdisciplinary research team. You will learn and apply different cellular, molecular, and biochemical assays to validate these findings. The candidate will also have the opportunity to develop and implement new protocols for gene-editing and mass spectrometry-based proteomics experiments.
Working as part of the infectious biology group in the Krogan lab, you will express affinity-tagged proteins of interest, perform affinity purifications, and proximity labeling of proteins and their interactors for mass spectrometry analysis to study protein-protein interactions. Additional major responsibilities will include the preparation, sequencing, and analysis of next-generation sequencing libraries to support CRISPR screening experiments. Further responsibilities will consist of isolation and culture of primary human immune cells, as well as functional analysis of engineered cells via Sanger sequencing and flow cytometry. The projects will also involve the purification of infectious HIV and infection of T cells with HIV in a biosafety level-3 facility. Strong organizational and communication skills are required.
The Krogan Lab is a highly dynamic and collaborative laboratory focusing on molecular networks underpinning infectious disease, carcinogenesis, and neurological disorders. Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently. Candidate's CV and/or cover letter must state basic qualifications (or if pending) upon submission. Candidates must meet all qualifications by the time of hire.
Required Qualifications:
Junior Specialist- a bachelor's degree (or equivalent degree) or at least four years of research experience.
Assistant Specialist- a master's degree or a bachelor's degree with three or more years of research experience.
Experience in the essential molecular biology techniques of molecular cloning, PCR, and
western blotting.
Experience in cell/tissue culture.
Able to work independently and meet deadlines; recognize and resolve problems while
maintaining a high level of organization.
Effective verbal and written communication skills to interface with the team to review and
analyze the results.
Willingness to work with infectious HIV virus in a BSL-3 facility
Willingness and ability to learn new methods and skills for changing research priorities
Excellent organizational and interpersonal communication skills (verbal and written)
Preferred Qualifications:
Experience transfection or viral transduction.
Experience with affinity purifications of proteins.
Experience with CRISPR/gene editing techniques
Experience with immunoprecipitation.
Experience in preparation of samples for mass spectrometric analysis.
Experience in fluorescence microscopy.
Experience with mass spectrometry data.
Experience with managing large datasets and/or databases.
Experience with statistical data analysis.
Please apply online at https://aprecruit.ucsf.edu/JPF04948, with a CV, cover letter, and three references.
See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$65,900.
Application Requirements
Document requirements
Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).
Cover Letter
Misc / Additional (Optional)
Reference requirements
3 required (contact information only)
About UC San Francisco
As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Job locationSan Francisco, CA