Valid8 Financial, Inc.
Senior Research Associate
Valid8 Financial, Inc., San Francisco, California, United States, 94199
Senior Research Associate
Molecular Biology and Expression, Protein Sciences
October 2024
Department:
Technology
Position Summary
TCG Labs-Soleil is seeking an experienced and motivated senior research associate with a passion for developing therapeutics to join our Protein Sciences team and execute efficient cloning and expression in support of a highly productive therapeutic biologics Research team. The successful candidate will have extensive skills in molecular biology and experience in expression of recombinant proteins using both transient and stable mammalian cell systems. As part of an interdisciplinary research team, the selected individual will be a key contributor to the progression of an exciting and diverse therapeutic pipeline.Responsibilities
Perform molecular biology techniques including high throughput PCR and molecular cloning, DNA mutagenesis, small to large scale plasmid DNA purificationDesign primers for DNA mutagenesis, cloning and sequence confirmationTrack expression plasmid lots and confirm identity by sequencingPerform standard cell culture support including maintenance of CHO and 293 cell linesPerform small to large scale transient expressions in CHO and 293 cellsPerform fed batch cultures of CHO stable cell lines to generate recombinant antibodiesHTP transient expression of antibodies in mammalian cell lines on automated platformDevelop and generate antibody stable expression cell lines in CHO and 293Determine expression titer of transient expressions and stable by BLI (96 or 384 well format)Accurately and consistently record experimental methods, materials, and resultsProvide regular progress updates and present data at internal team meetings and departmental meetingsQualificationsM.S. in Molecular Biology, Chemical Biology, Biochemistry or other relevant discipline with 5+ years of relevant experience, or B.S. with equivalent experienceSelf-motivated and passionate about automation and new technologyExperienced with various molecular cloning methods such as restriction cloning, seamless (Gibson Assembly) and Golden Gate cloning is requiredExtensive hands-on experience with primer design and PCR is requiredMammalian cell culture experience is requiredExtensive hands-on experience with transient mammalian expression (293 and CHO) is highly desired.Hands-on experience with Molecular Biology and Sequencing as well as data analysis and visualization software is desired (i.e. Geneious, Graphpad Prism)Hands-on experience of mono- and bi-specific antibodies, Fab fragments, scFv, VHH, and various antibody-fusion formats is a plusHands on experience with automated platforms is desiredStrong organizational skills and ability to handle multiple projects simultaneously and ability to work in a flexible-team oriented environmentExperienced with cross functional collaborations and comfortable with a fast-paced work environment.Company OverviewTCG Labs-Soleil, established in partnership with The Column Group, a science-focused venture capital firm, is pioneering a novel ‘venture capital-biotech’ model. Our core mission is to efficiently translate scientific insights into therapeutic solutions for patients facing serious diseases. This model supports scientific entrepreneurs from the initial idea to clinical proof of concept (PoC) by combining a dedicated fund (TCG Labs) with our independent, evergreen R&D center led by a top-tier scientific team (Soleil). Together, we create an integrated ecosystem that fosters the development of single-asset portfolio companies, strategically poised for transacting after clinical PoC.TCG Labs-Soleil is an Equal Opportunity Employer. We do not discriminate against qualified applicants based upon any protected group status, including but not limited to race, religion, color, creed, sex (including breastfeeding and related conditions), gender identity/gender expression, pregnancy (including childbirth and related conditions), national origin, ancestry, age, marital status, military or veteran status, sexual orientation, genetic information, physical or mental disability or medical condition as defined by applicable equal opportunity laws. Any request for accommodation can be directed to contact@tcglsoleil.com.Benefits and CompensationTCG Labs-Soleil provides a competitive benefits package that includes access to Medical, Dental, and Vision Insurance, Life Insurance, Short and Long Term Disability, Health Savings Account (HSA), Flexible Spending Accounts (FSA), 401k plan and PTO.The anticipated salary range for fully qualified candidates applying for this role will be $80,500 - $143,750 annually (not including discretionary bonus, equity, and benefits). The final salary offered will be dependent on several factors that include but are not limited to relevant previous experience including duration and type of experience within the industry, education, and internal equity.
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Molecular Biology and Expression, Protein Sciences
October 2024
Department:
Technology
Position Summary
TCG Labs-Soleil is seeking an experienced and motivated senior research associate with a passion for developing therapeutics to join our Protein Sciences team and execute efficient cloning and expression in support of a highly productive therapeutic biologics Research team. The successful candidate will have extensive skills in molecular biology and experience in expression of recombinant proteins using both transient and stable mammalian cell systems. As part of an interdisciplinary research team, the selected individual will be a key contributor to the progression of an exciting and diverse therapeutic pipeline.Responsibilities
Perform molecular biology techniques including high throughput PCR and molecular cloning, DNA mutagenesis, small to large scale plasmid DNA purificationDesign primers for DNA mutagenesis, cloning and sequence confirmationTrack expression plasmid lots and confirm identity by sequencingPerform standard cell culture support including maintenance of CHO and 293 cell linesPerform small to large scale transient expressions in CHO and 293 cellsPerform fed batch cultures of CHO stable cell lines to generate recombinant antibodiesHTP transient expression of antibodies in mammalian cell lines on automated platformDevelop and generate antibody stable expression cell lines in CHO and 293Determine expression titer of transient expressions and stable by BLI (96 or 384 well format)Accurately and consistently record experimental methods, materials, and resultsProvide regular progress updates and present data at internal team meetings and departmental meetingsQualificationsM.S. in Molecular Biology, Chemical Biology, Biochemistry or other relevant discipline with 5+ years of relevant experience, or B.S. with equivalent experienceSelf-motivated and passionate about automation and new technologyExperienced with various molecular cloning methods such as restriction cloning, seamless (Gibson Assembly) and Golden Gate cloning is requiredExtensive hands-on experience with primer design and PCR is requiredMammalian cell culture experience is requiredExtensive hands-on experience with transient mammalian expression (293 and CHO) is highly desired.Hands-on experience with Molecular Biology and Sequencing as well as data analysis and visualization software is desired (i.e. Geneious, Graphpad Prism)Hands-on experience of mono- and bi-specific antibodies, Fab fragments, scFv, VHH, and various antibody-fusion formats is a plusHands on experience with automated platforms is desiredStrong organizational skills and ability to handle multiple projects simultaneously and ability to work in a flexible-team oriented environmentExperienced with cross functional collaborations and comfortable with a fast-paced work environment.Company OverviewTCG Labs-Soleil, established in partnership with The Column Group, a science-focused venture capital firm, is pioneering a novel ‘venture capital-biotech’ model. Our core mission is to efficiently translate scientific insights into therapeutic solutions for patients facing serious diseases. This model supports scientific entrepreneurs from the initial idea to clinical proof of concept (PoC) by combining a dedicated fund (TCG Labs) with our independent, evergreen R&D center led by a top-tier scientific team (Soleil). Together, we create an integrated ecosystem that fosters the development of single-asset portfolio companies, strategically poised for transacting after clinical PoC.TCG Labs-Soleil is an Equal Opportunity Employer. We do not discriminate against qualified applicants based upon any protected group status, including but not limited to race, religion, color, creed, sex (including breastfeeding and related conditions), gender identity/gender expression, pregnancy (including childbirth and related conditions), national origin, ancestry, age, marital status, military or veteran status, sexual orientation, genetic information, physical or mental disability or medical condition as defined by applicable equal opportunity laws. Any request for accommodation can be directed to contact@tcglsoleil.com.Benefits and CompensationTCG Labs-Soleil provides a competitive benefits package that includes access to Medical, Dental, and Vision Insurance, Life Insurance, Short and Long Term Disability, Health Savings Account (HSA), Flexible Spending Accounts (FSA), 401k plan and PTO.The anticipated salary range for fully qualified candidates applying for this role will be $80,500 - $143,750 annually (not including discretionary bonus, equity, and benefits). The final salary offered will be dependent on several factors that include but are not limited to relevant previous experience including duration and type of experience within the industry, education, and internal equity.
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