Scientist Cellular Genomics

Job Title: Scientist Cellular Genomics Location: Cambridge, MA Type: Contract Responsibilities Follow existing lab standard operating procedures to: Participate in sample collection of murine and human tissues, including the preparation of tubes, interfacing with in vivo scientists to retrieve samples, isolation of primary immune cells, and standard mammalian cell culture Participate in sample preparation of murine and human tissues, including tissue fixation, dissociation, sectioning, and antibody staining Generate spatial and single-cell data via a number of commercial workflows, including 10x Genomics and Parse Technologies Interpret quality control metrics for spatial, single-cell and cytometry experiments Requirements MA + 3 years of experience in Biology, Immunology, or a related field 3+ years of hands-on experience with standard immunoprofiling technologies, including Flow Cytometry Single-cell library preparation (ideally 10x Genomics or Parse Technology) Preparation and loading of Illumina sequencing libraries Bonus: generation of spatial transcriptomics data (any technology) 3+ years of experience with primary immune cells, including Primary immune cell isolation Maintenance of immune cell lines Cell culture and in vitro stimulation of primary immune cells Bonus: experience with CRISPR mediated functional genomics, including electroporation of primary immune cells Benefits System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.