University of California - San Francisco
Junior/Assistant/Associate Specialist (Protein Engineering)
University of California - San Francisco, San Francisco, California, United States, 94199
Application Window
Open date:
February 21, 2024Next review date:
Thursday, Mar 7, 2024 at 11:59pm (Pacific Time)Apply by this date to ensure full consideration by the committee.Final date:
Thursday, Aug 21, 2025 at 11:59pm (Pacific Time)Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.Position Description
Junior/Assistant/Associate Specialist (Protein Engineering)Search# JPF04935Antibiome Center, Department of Pharmaceutical Chemistry UCSFThe Antibiome Center is seeking a highly motivated candidate to join our quick-paced and multi-talented team to develop antibodies at the proteome-wide scale. The successful candidate will be responsible for molecular biology, generation of antibody-display library, protein-antibody expression and purification, and protein-antibody validation. The candidate will have opportunities to learn state-of-the-art techniques and automation systems. Our laboratory is highly collaborative and is engaged in numerous academic and industrial collaborative projects.The Antibiome Center is part of the Recombinant Antibody Network (RAN), a collaborative consortium consisting of laboratories at the University of California San Francisco (Dr. Jim Wells), University of Chicago (Dr. Tony Kossiakoff), and the University of Toronto (Dr. Sachdev Sidhu). We are committed to generating recombinant antibodies at the surface proteome-wide scale using high throughput phage display approaches. Our long history of protein engineering expertise also enables the development of bio-therapeutic antibodies in the immuno-oncology field.Primary Responsibilities:Cloning recombinant protein and antibody constructs (including reformatting to scFv, bispecifics) using a variety of molecular biology techniques including Polymerase Chain Reaction, Gibson assembly, DNA purification, and transformation.Antibody display library generation (phage, yeast, or mammalian) including library design (Kunkel mutagenesis, Golden Gate Assembly, Lentivirus production).Protein expression from E. coli and mammalian sources (HEK293 and CHO cells).Purification of proteins using various chromatography techniques such as affinity with Protein A, IMAC with Ni-NTA, and IEX.Characterization of proteins by a variety of techniques including SDS-PAGE, ELISA, SEC, and Western Blotting.Required Qualifications:Junior Specialist- A B.S. degree (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or at least four years of research experience.Assistant Specialist- A master's degree in biochemistry, cell biology, or relevant life sciences field or a bachelor's degree with 3 or more years of research experience.Associate rank- a master's degree (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or five to ten years of research experience.Candidates must meet the required qualifications at the time of appointment. Candidate's CV must state qualifications (or if pending) upon submission.Preferred Qualifications:Expertise in display library generation and selection.Experience with Next-Generation-Sequencing techniques and analysis.Familiarity with mammalian tissue culture techniques including transfections, cell line maintenance, recombinant protein expression.Proficiency with advanced biophysical techniques such as Biolayer Interferometry (BLI), Surface Plasmon Resonance (SPR), poly-reactivity assays, HPLC chromatography.Data analysis experience using programming languages such as R or Python.Junior/Assistant Specialist- at least one year of wet laboratory experience.Please apply online at https://aprecruit.ucsf.edu/JPF04935, with a CV, cover letter, and three references.See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$82,100.Application Requirements
Document Requirements:Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).Cover LetterMisc / Additional (Optional)Reference Requirements:3 required (contact information only)About UC San Francisco
As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.Job Location
San Francisco, CA
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Open date:
February 21, 2024Next review date:
Thursday, Mar 7, 2024 at 11:59pm (Pacific Time)Apply by this date to ensure full consideration by the committee.Final date:
Thursday, Aug 21, 2025 at 11:59pm (Pacific Time)Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.Position Description
Junior/Assistant/Associate Specialist (Protein Engineering)Search# JPF04935Antibiome Center, Department of Pharmaceutical Chemistry UCSFThe Antibiome Center is seeking a highly motivated candidate to join our quick-paced and multi-talented team to develop antibodies at the proteome-wide scale. The successful candidate will be responsible for molecular biology, generation of antibody-display library, protein-antibody expression and purification, and protein-antibody validation. The candidate will have opportunities to learn state-of-the-art techniques and automation systems. Our laboratory is highly collaborative and is engaged in numerous academic and industrial collaborative projects.The Antibiome Center is part of the Recombinant Antibody Network (RAN), a collaborative consortium consisting of laboratories at the University of California San Francisco (Dr. Jim Wells), University of Chicago (Dr. Tony Kossiakoff), and the University of Toronto (Dr. Sachdev Sidhu). We are committed to generating recombinant antibodies at the surface proteome-wide scale using high throughput phage display approaches. Our long history of protein engineering expertise also enables the development of bio-therapeutic antibodies in the immuno-oncology field.Primary Responsibilities:Cloning recombinant protein and antibody constructs (including reformatting to scFv, bispecifics) using a variety of molecular biology techniques including Polymerase Chain Reaction, Gibson assembly, DNA purification, and transformation.Antibody display library generation (phage, yeast, or mammalian) including library design (Kunkel mutagenesis, Golden Gate Assembly, Lentivirus production).Protein expression from E. coli and mammalian sources (HEK293 and CHO cells).Purification of proteins using various chromatography techniques such as affinity with Protein A, IMAC with Ni-NTA, and IEX.Characterization of proteins by a variety of techniques including SDS-PAGE, ELISA, SEC, and Western Blotting.Required Qualifications:Junior Specialist- A B.S. degree (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or at least four years of research experience.Assistant Specialist- A master's degree in biochemistry, cell biology, or relevant life sciences field or a bachelor's degree with 3 or more years of research experience.Associate rank- a master's degree (or equivalent degree) in biochemistry, cell biology, or relevant life sciences field or five to ten years of research experience.Candidates must meet the required qualifications at the time of appointment. Candidate's CV must state qualifications (or if pending) upon submission.Preferred Qualifications:Expertise in display library generation and selection.Experience with Next-Generation-Sequencing techniques and analysis.Familiarity with mammalian tissue culture techniques including transfections, cell line maintenance, recombinant protein expression.Proficiency with advanced biophysical techniques such as Biolayer Interferometry (BLI), Surface Plasmon Resonance (SPR), poly-reactivity assays, HPLC chromatography.Data analysis experience using programming languages such as R or Python.Junior/Assistant Specialist- at least one year of wet laboratory experience.Please apply online at https://aprecruit.ucsf.edu/JPF04935, with a CV, cover letter, and three references.See Table 24B for the salary range for this position. A reasonable estimate for this position is $51,300-$82,100.Application Requirements
Document Requirements:Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).Cover LetterMisc / Additional (Optional)Reference Requirements:3 required (contact information only)About UC San Francisco
As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.Job Location
San Francisco, CA
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