National Institute for Bioprocessing Research and...
Postdoctoral Researcher
National Institute for Bioprocessing Research and..., Dublin, Indiana, us, 47335
Research
Vacancy Summary
This is an exciting, 5-year opportunity for an experienced Postdoctoral Researcher to work on, and influence, a research programme funded through a prestigious SFI Research Professorship award to Professor Mark Smales in the area of enhancing advanced biotherapeutic biological medicines design, manufacturing and activity at NIBRT. The role will help lead and deliver a work programme to generate a fundamental, transformative and conceptual mechanistic shift in our understanding of the cell expression systems and processes used to produce these advanced biotherapeutics, and apply modern engineering biology approaches to deliver more efficient, predictable, and affordable manufacturing approaches. The postholder will be expected to (1) undertake the work and training programme outlined in the grant proposal ‘Enhancing Advanced Biotherapeutic Design, Manufacturing and Activity: Defining and Leveraging the Biological Rules that Underpin AAV Gene Therapy, IVTmRNA and Protein Biotherapeutic Modality Design and Production’ for PDR1, and (2) provide support and supervision of other members of Prof Smales team.
Position Available
Postdoctoral Researcher (PDR)/Research Scientist L2
Location
Dublin, Ireland
Department
Research
Hiring Manager
Prof Mark Smales
Contract Type
5 year Specific Purpose Contract
Application Date
ASAP
Interview Dates
TBC
Salary & Benefits
Competitive salary and excellent suite of benefits including pension, private health insurance, income protection scheme, 25 days annual leave plus up to 3 additional company days off, generous training and career development supports, flexible working hours and work from home set up supported.
Role Purpose
The main focus of the work for PDR1 will be to work on the development of a better understanding of the biology of HEK293 cells and genetic components of the target genome that underpin rAAV vector production and apply this to generate new HEK293 hosts, bioprocesses and vectors for improved manufacturing. Specific objectives are to deliver: (1) a fundamental understanding of the impact of rAAV production on the HEK293 cell factory and its ability to produce rAAV at high yield and quality; (2) determine the cellular constraints in HEK293 cells and systems that limit production of functional (fully packaged) rAAV vectors; (3) utilise cell engineering and synthetic biology approaches to generate new HEK293 cell hosts that aid intensification of cell bioprocesses to enhance yield of rAAV, and (4) manipulate genome genetic elements to tune their expression in a tissue specific manner.
Key Responsibilities
The key responsibilities of the job include:
To devise, undertake appropriate experiments, interpret the findings and devise new approaches to achieve the goals and milestones as described in the Research Professorship SFI funded grant programme.
To undertake the mandatory training, reporting, public engagement/outreach activities and any other requirements outlined under the funding agreement for the project.
Be responsible for maintenance of laboratory records, health and safety records, and inventory around the project.
To interact with other collaborators at NIBRT and externally, both within Ireland and internationally, coordinating activity across the consortium.
Regularly report to the supervisors and consortium, compile data acquired in a form to allow rapid transformation of documented research results into high quality research papers and/or patent applications.
Attend and present at training activities and research summits and meetings.
Provide training support and advice to graduate, postdoctoral scientists, technicians and other staff at NIBRT.
Write up research findings for publication in leading peer-reviewed international journals.
Be committed to your own personal career development.
Other duties, commensurate with the grading of the job, as assigned from time to time.
Essential Criteria
A PhD in biotechnology, biochemistry, biochemical engineering, genetics, molecular biology, gene therapy, engineering biology, synthetic biology, RNA sequencing or a relevant discipline.
Training/experience of working with mammalian cells in culture and expression of exogenous genes and recombinant proteins.
Experience of molecular biology and the designing and generation of expression vectors.
Training and experience in DNA, mRNA and protein analysis.
Experience of project management.
Desirable Criteria
Experience of working with AAV gene therapy manufacturing and mammalian cell expression hosts for their production, particularly HEK293 cells.
Previous postdoctoral experience in an area relevant to those areas and disciplines outlined in (1) above.
Experience of RNA sequencing and bioinformatic analysis.
Knowledge and experience of running Ambr fermentation experiments.
Knowledge of the wider bioprocessing field, particularly the growing portfolio of advanced biotherapies.
Experience of bioengineering/synthetic biology tuning of mammalian cells.
Knowledge and experience of working on mRNA translation and its control.
Required Skills & Competencies
Ability to design and execute experiments on an independent basis.
Experience of writing publications for scientific journals and scientific reports.
Experience of supervision and training of others and of working in collaborative teams.
How To Apply?
To apply for this position please forward your CV and cover letter to careers@nibrt.ie
Please include your name and the position which you are applying for in the subject line.
Informal enquiries can be made in confidence by contacting careers@nibrt.ie
NIBRT is an Equal Opportunities Employer.
#J-18808-Ljbffr
Vacancy Summary
This is an exciting, 5-year opportunity for an experienced Postdoctoral Researcher to work on, and influence, a research programme funded through a prestigious SFI Research Professorship award to Professor Mark Smales in the area of enhancing advanced biotherapeutic biological medicines design, manufacturing and activity at NIBRT. The role will help lead and deliver a work programme to generate a fundamental, transformative and conceptual mechanistic shift in our understanding of the cell expression systems and processes used to produce these advanced biotherapeutics, and apply modern engineering biology approaches to deliver more efficient, predictable, and affordable manufacturing approaches. The postholder will be expected to (1) undertake the work and training programme outlined in the grant proposal ‘Enhancing Advanced Biotherapeutic Design, Manufacturing and Activity: Defining and Leveraging the Biological Rules that Underpin AAV Gene Therapy, IVTmRNA and Protein Biotherapeutic Modality Design and Production’ for PDR1, and (2) provide support and supervision of other members of Prof Smales team.
Position Available
Postdoctoral Researcher (PDR)/Research Scientist L2
Location
Dublin, Ireland
Department
Research
Hiring Manager
Prof Mark Smales
Contract Type
5 year Specific Purpose Contract
Application Date
ASAP
Interview Dates
TBC
Salary & Benefits
Competitive salary and excellent suite of benefits including pension, private health insurance, income protection scheme, 25 days annual leave plus up to 3 additional company days off, generous training and career development supports, flexible working hours and work from home set up supported.
Role Purpose
The main focus of the work for PDR1 will be to work on the development of a better understanding of the biology of HEK293 cells and genetic components of the target genome that underpin rAAV vector production and apply this to generate new HEK293 hosts, bioprocesses and vectors for improved manufacturing. Specific objectives are to deliver: (1) a fundamental understanding of the impact of rAAV production on the HEK293 cell factory and its ability to produce rAAV at high yield and quality; (2) determine the cellular constraints in HEK293 cells and systems that limit production of functional (fully packaged) rAAV vectors; (3) utilise cell engineering and synthetic biology approaches to generate new HEK293 cell hosts that aid intensification of cell bioprocesses to enhance yield of rAAV, and (4) manipulate genome genetic elements to tune their expression in a tissue specific manner.
Key Responsibilities
The key responsibilities of the job include:
To devise, undertake appropriate experiments, interpret the findings and devise new approaches to achieve the goals and milestones as described in the Research Professorship SFI funded grant programme.
To undertake the mandatory training, reporting, public engagement/outreach activities and any other requirements outlined under the funding agreement for the project.
Be responsible for maintenance of laboratory records, health and safety records, and inventory around the project.
To interact with other collaborators at NIBRT and externally, both within Ireland and internationally, coordinating activity across the consortium.
Regularly report to the supervisors and consortium, compile data acquired in a form to allow rapid transformation of documented research results into high quality research papers and/or patent applications.
Attend and present at training activities and research summits and meetings.
Provide training support and advice to graduate, postdoctoral scientists, technicians and other staff at NIBRT.
Write up research findings for publication in leading peer-reviewed international journals.
Be committed to your own personal career development.
Other duties, commensurate with the grading of the job, as assigned from time to time.
Essential Criteria
A PhD in biotechnology, biochemistry, biochemical engineering, genetics, molecular biology, gene therapy, engineering biology, synthetic biology, RNA sequencing or a relevant discipline.
Training/experience of working with mammalian cells in culture and expression of exogenous genes and recombinant proteins.
Experience of molecular biology and the designing and generation of expression vectors.
Training and experience in DNA, mRNA and protein analysis.
Experience of project management.
Desirable Criteria
Experience of working with AAV gene therapy manufacturing and mammalian cell expression hosts for their production, particularly HEK293 cells.
Previous postdoctoral experience in an area relevant to those areas and disciplines outlined in (1) above.
Experience of RNA sequencing and bioinformatic analysis.
Knowledge and experience of running Ambr fermentation experiments.
Knowledge of the wider bioprocessing field, particularly the growing portfolio of advanced biotherapies.
Experience of bioengineering/synthetic biology tuning of mammalian cells.
Knowledge and experience of working on mRNA translation and its control.
Required Skills & Competencies
Ability to design and execute experiments on an independent basis.
Experience of writing publications for scientific journals and scientific reports.
Experience of supervision and training of others and of working in collaborative teams.
How To Apply?
To apply for this position please forward your CV and cover letter to careers@nibrt.ie
Please include your name and the position which you are applying for in the subject line.
Informal enquiries can be made in confidence by contacting careers@nibrt.ie
NIBRT is an Equal Opportunities Employer.
#J-18808-Ljbffr