Case Western Reserve University
Post-doctoral Fellow - Medicine / Institute for Transformative Molecular Medicin
Case Western Reserve University, Baltimore, Maryland, United States,
Post-doctoral Fellow - Medicine / Institute for Transformative Molecular Medicine
Posted: April 11, 2024Department: Medicine / Institute for Transformative Molecular MedicineSupervisor Name and Title: Jonathan Stamler, MD, Professor of Medicine; Director, Institute for Transformative Molecular MedicinePOSITION OBJECTIVEThe NIH-funded project examines protein-S-nitrosylation in regulating G protein-coupled receptor signaling pathways in the lung in health and in models of asthma. Desired expertise includes experience with in vivo physiology of mouse lung and mouse physiology assessments (ideally using flexiVent or other ventilator), GPCR signaling pathway analysis in vivo and in model cell systems, and biochemical/enzyme/proteomic methods. The Stamler lab is a dynamic environment engaged in a wide array of basic and translational studies seeking to understand protein S-nitrosylation as an enzymatically-regulated post-translational modification that alters protein functions broadly in physiology and in pathophysiology/disease, and to manipulate this for therapeutic benefit.ESSENTIAL FUNCTIONSCoordinate laboratory research activities. Follow approved animal protocol and standard workflow to induce asthma-like state in mice. Perform mouse surgery to install ventilator system, and measure airway reaction to bronchoconstrictor and bronchorelaxant drugs to quantify lung dysfunction.Isolate bronchoalveolar lavage fluid for assessment of immune cell infiltration into lung. Isolate mouse tissues to perform histology staining for lung integrity and biochemical assays to measure protein levels (western blot) and S-nitroso-protein levels (SNO-RAC).Utilize samples from human primary airway epithelial and smooth muscle cells to validate findings in mice.Maintain ongoing records of work and analysis performed (lab notebook) and maintain archive of all data generated.Work closely with the principal investigator and with supervising investigator, keeping them informed of progress and difficulties.Evaluate adequacy of techniques; study and test new procedures and analyze data.Supervise students and technicians, specifically training in techniques and overseeing their use.Organize overall research work and recent progress in summary presentations for oral/visual presentation to local research group and to the off-site members of the PPG.May co-author research projects, providing assistance in data analysis and interpretation, in detailed recordkeeping of procedures and results, and in written descriptions of methods and results in manuscripts.NONESSENTIAL FUNCTIONSPerform other duties as assigned.CONTACTSDaily contact with PI and/or direct supervisor to discuss research and maintain workflow. Weekly research group lab meetings with other lab members. Rare contact with other departments to share information. Regular contact with other members of multisite PPG group to exchange information and with PPG core facilities as needed.SUPERVISORY RESPONSIBILITYMay supervise students and technicians.QUALIFICATIONSExperience: 3 to 5 years of related research experience required. Rodent handling experience for at least 1 year strongly recommended. Experience with rodent asthma models, mouse surgery, and flexiVent or similar ventilator system is a strong plus.Education/Licensing: Ph.D. degree in a natural science-relevant field required.REQUIRED SKILLSKnowledge of commonly-used concepts, practices, and procedures in biochemistry (particularly western blotting), in animal handling, physiological assessment and surgery (flexi-Vent experience a plus), and in asthma models in mice.Ability to act independently on general instructions to perform the functions of the job.Ability to operate laboratory equipment.Ability to meet consistent attendance.Ability to interact with colleagues, supervisors and customers face to face.WORKING CONDITIONSGeneral laboratory environment. Will work extensively with laboratory mice. May have exposure to hazards, particularly including dust mite allergens used for allergic asthma modeling in mice.APPLICANT INSTRUCTIONSInterested candidates should send a cover/motivation letter and a CV with the names and contact information of 2-3 references to Justin Donnelly (jcd109@case.edu).
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Posted: April 11, 2024Department: Medicine / Institute for Transformative Molecular MedicineSupervisor Name and Title: Jonathan Stamler, MD, Professor of Medicine; Director, Institute for Transformative Molecular MedicinePOSITION OBJECTIVEThe NIH-funded project examines protein-S-nitrosylation in regulating G protein-coupled receptor signaling pathways in the lung in health and in models of asthma. Desired expertise includes experience with in vivo physiology of mouse lung and mouse physiology assessments (ideally using flexiVent or other ventilator), GPCR signaling pathway analysis in vivo and in model cell systems, and biochemical/enzyme/proteomic methods. The Stamler lab is a dynamic environment engaged in a wide array of basic and translational studies seeking to understand protein S-nitrosylation as an enzymatically-regulated post-translational modification that alters protein functions broadly in physiology and in pathophysiology/disease, and to manipulate this for therapeutic benefit.ESSENTIAL FUNCTIONSCoordinate laboratory research activities. Follow approved animal protocol and standard workflow to induce asthma-like state in mice. Perform mouse surgery to install ventilator system, and measure airway reaction to bronchoconstrictor and bronchorelaxant drugs to quantify lung dysfunction.Isolate bronchoalveolar lavage fluid for assessment of immune cell infiltration into lung. Isolate mouse tissues to perform histology staining for lung integrity and biochemical assays to measure protein levels (western blot) and S-nitroso-protein levels (SNO-RAC).Utilize samples from human primary airway epithelial and smooth muscle cells to validate findings in mice.Maintain ongoing records of work and analysis performed (lab notebook) and maintain archive of all data generated.Work closely with the principal investigator and with supervising investigator, keeping them informed of progress and difficulties.Evaluate adequacy of techniques; study and test new procedures and analyze data.Supervise students and technicians, specifically training in techniques and overseeing their use.Organize overall research work and recent progress in summary presentations for oral/visual presentation to local research group and to the off-site members of the PPG.May co-author research projects, providing assistance in data analysis and interpretation, in detailed recordkeeping of procedures and results, and in written descriptions of methods and results in manuscripts.NONESSENTIAL FUNCTIONSPerform other duties as assigned.CONTACTSDaily contact with PI and/or direct supervisor to discuss research and maintain workflow. Weekly research group lab meetings with other lab members. Rare contact with other departments to share information. Regular contact with other members of multisite PPG group to exchange information and with PPG core facilities as needed.SUPERVISORY RESPONSIBILITYMay supervise students and technicians.QUALIFICATIONSExperience: 3 to 5 years of related research experience required. Rodent handling experience for at least 1 year strongly recommended. Experience with rodent asthma models, mouse surgery, and flexiVent or similar ventilator system is a strong plus.Education/Licensing: Ph.D. degree in a natural science-relevant field required.REQUIRED SKILLSKnowledge of commonly-used concepts, practices, and procedures in biochemistry (particularly western blotting), in animal handling, physiological assessment and surgery (flexi-Vent experience a plus), and in asthma models in mice.Ability to act independently on general instructions to perform the functions of the job.Ability to operate laboratory equipment.Ability to meet consistent attendance.Ability to interact with colleagues, supervisors and customers face to face.WORKING CONDITIONSGeneral laboratory environment. Will work extensively with laboratory mice. May have exposure to hazards, particularly including dust mite allergens used for allergic asthma modeling in mice.APPLICANT INSTRUCTIONSInterested candidates should send a cover/motivation letter and a CV with the names and contact information of 2-3 references to Justin Donnelly (jcd109@case.edu).
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