Salk Institute for Biological Studies
Lab Manager Laboratory of Dr. Alan Saghatelian
Salk Institute for Biological Studies, San Diego, California, United States, 92189
Job location is at
10010 N Torrey Pines Rd, La Jolla, CA 92037The lab manager position is dual in scope: as a bench scientist supporting studies in the lab and as a laboratory manager. Alan Saghatelian's laboratory uses next-generation nucleotide sequencing and mass spectrometry to identify novel microproteins and define their functions in cells and in mice. This position places significant emphasis on the bench scientist role. The candidate will spend most of their time using cell and molecular biology techniques to support peptide discovery projects from hit identification to validation and functional studies. Lab manager duties will include ordering supplies, managing the database of lab materials, and assisting the PI with budgeting. The candidate will also supervise and train junior technicians, students, and postdoctoral fellows in molecular and cell biology techniques.Who We AreThe Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community comprises a diverse and talented group of individuals, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.What Your Key Responsibilities Will Be1) Research Support (70%)The candidate will use a variety of molecular biology techniques, including designing DNA primers for PCR, ordering DNA constructs and plasmids, and confirming their fidelity using restriction enzymes and gel electrophoresis. The candidate should be familiar with Gateway and Gibson cloning methods and software such as SnapGene.Responsible for routinely growing cells in culture, transfecting cells with plasmids to express proteins of interest, and establishing stable cell lines. Studies may require measuring cell secretion products using commercial ELISAs.Use cell surface receptor signaling assays (time-resolved fluorescence and luciferase reporter) to determine the structural requirements of microproteins for binding and activation.Review relevant literature to solve problems and modify and adapt techniques to implement new protocols for lab studies.Use plate-based fluorescent and luminescent activity assays for in-vitro pharmacology of various targets.Develop and execute experiments involving PCR, sequencing, and other DNA-based techniques.Prepare cells and tissues for RNAseq and Riboseq analysis and maintain the workflow for submission to Institute core facilities for sequencing.Assist post-doctoral fellows and provide technical expertise, including carrying out CRISPR screens by preparing lentiviral libraries and cell selection or FACS.2) Laboratory Management (20%)Manage the ordering of reagents and supplies for lab projects using purchasing software. Approve all orders and, together with the PI, manage the funding to be used.Manage freezer inventory and databases of plasmids and reagents in the lab in electronic format.Oversee general lab maintenance and safety. Inform new personnel of required safety procedures and ensure safety updates are disseminated to laboratory personnel.With assistance from postdoctoral fellows, train and oversee junior technicians and undergraduate students in molecular and cell biology techniques.Communicate and interact with Salk departments (HR, accounting, environmental health, materials transfer) and external vendors to conduct necessary lab business.3) Other Duties as Assigned (10%)Analyze data, prepare graphics using standard programs and present in group meetings.Ensure quality and integrity of data collected and maintain data in electronic form.Assist PIs and postdocs with grant and report writing and study-related publications.Supervisory Responsibilities:This job has no supervisory responsibilities.What we RequireBachelor's degree in biology or related field.Minimum of 5 years of advanced experience in molecular and cell biology, including design of DNA constructs and sequencing and in-vitro cell-based assays with ELISAs or CRE-luciferase or time-resolved fluorescence readouts.Minimum 3 years of experience in a supervisory/managerial role within a laboratory setting.Strong collaborative and written and verbal communication skills.Preferred:Experience with receptor signaling assays. Experience with the preparation of samples for riboseq or RNAseq.What We Can OfferThe expected pay range for this position is $82,000 to $102,750 annually.
Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.BenefitsSalk Institute offers competitive benefits, including medical, dental, vision, retirement, paid time off, tuition reimbursement, patient advocacy services, and transit/parking program.Equal Employment Opportunity StatementThe Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students, employees, applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace.Accordingly, the Institute prohibits harassment and discrimination in employment on the basis of, and considers all qualified applicants for employment without regard to, actual or perceived race, color, religion, religious creed (including religious dress and grooming practices), national origin, ancestry, citizenship, physical or mental disability, medical condition (including cancer and genetic characteristics), genetic information, marital status, age, sex (including pregnancy, childbirth, breastfeeding, or related medical conditions), gender, gender identity, gender expression, sexual orientation, veteran and/or military status (disabled veteran, veteran of the Vietnam era, other covered veteran status), political affiliation, and any other status protected by state or federal law.
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10010 N Torrey Pines Rd, La Jolla, CA 92037The lab manager position is dual in scope: as a bench scientist supporting studies in the lab and as a laboratory manager. Alan Saghatelian's laboratory uses next-generation nucleotide sequencing and mass spectrometry to identify novel microproteins and define their functions in cells and in mice. This position places significant emphasis on the bench scientist role. The candidate will spend most of their time using cell and molecular biology techniques to support peptide discovery projects from hit identification to validation and functional studies. Lab manager duties will include ordering supplies, managing the database of lab materials, and assisting the PI with budgeting. The candidate will also supervise and train junior technicians, students, and postdoctoral fellows in molecular and cell biology techniques.Who We AreThe Salk Institute is an internationally renowned research institution that values diversity, equity, and inclusion. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces diverse perspectives and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community comprises a diverse and talented group of individuals, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.What Your Key Responsibilities Will Be1) Research Support (70%)The candidate will use a variety of molecular biology techniques, including designing DNA primers for PCR, ordering DNA constructs and plasmids, and confirming their fidelity using restriction enzymes and gel electrophoresis. The candidate should be familiar with Gateway and Gibson cloning methods and software such as SnapGene.Responsible for routinely growing cells in culture, transfecting cells with plasmids to express proteins of interest, and establishing stable cell lines. Studies may require measuring cell secretion products using commercial ELISAs.Use cell surface receptor signaling assays (time-resolved fluorescence and luciferase reporter) to determine the structural requirements of microproteins for binding and activation.Review relevant literature to solve problems and modify and adapt techniques to implement new protocols for lab studies.Use plate-based fluorescent and luminescent activity assays for in-vitro pharmacology of various targets.Develop and execute experiments involving PCR, sequencing, and other DNA-based techniques.Prepare cells and tissues for RNAseq and Riboseq analysis and maintain the workflow for submission to Institute core facilities for sequencing.Assist post-doctoral fellows and provide technical expertise, including carrying out CRISPR screens by preparing lentiviral libraries and cell selection or FACS.2) Laboratory Management (20%)Manage the ordering of reagents and supplies for lab projects using purchasing software. Approve all orders and, together with the PI, manage the funding to be used.Manage freezer inventory and databases of plasmids and reagents in the lab in electronic format.Oversee general lab maintenance and safety. Inform new personnel of required safety procedures and ensure safety updates are disseminated to laboratory personnel.With assistance from postdoctoral fellows, train and oversee junior technicians and undergraduate students in molecular and cell biology techniques.Communicate and interact with Salk departments (HR, accounting, environmental health, materials transfer) and external vendors to conduct necessary lab business.3) Other Duties as Assigned (10%)Analyze data, prepare graphics using standard programs and present in group meetings.Ensure quality and integrity of data collected and maintain data in electronic form.Assist PIs and postdocs with grant and report writing and study-related publications.Supervisory Responsibilities:This job has no supervisory responsibilities.What we RequireBachelor's degree in biology or related field.Minimum of 5 years of advanced experience in molecular and cell biology, including design of DNA constructs and sequencing and in-vitro cell-based assays with ELISAs or CRE-luciferase or time-resolved fluorescence readouts.Minimum 3 years of experience in a supervisory/managerial role within a laboratory setting.Strong collaborative and written and verbal communication skills.Preferred:Experience with receptor signaling assays. Experience with the preparation of samples for riboseq or RNAseq.What We Can OfferThe expected pay range for this position is $82,000 to $102,750 annually.
Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs.BenefitsSalk Institute offers competitive benefits, including medical, dental, vision, retirement, paid time off, tuition reimbursement, patient advocacy services, and transit/parking program.Equal Employment Opportunity StatementThe Salk Institute for Biological Studies is an Equal Opportunity Employer and is committed to providing equal access to opportunities for students, employees, applicants for employment and other visitors. Salk has also adopted and maintains a policy to encourage professional and respectful workplace behavior and prevent discriminatory and harassing conduct in our workplace.Accordingly, the Institute prohibits harassment and discrimination in employment on the basis of, and considers all qualified applicants for employment without regard to, actual or perceived race, color, religion, religious creed (including religious dress and grooming practices), national origin, ancestry, citizenship, physical or mental disability, medical condition (including cancer and genetic characteristics), genetic information, marital status, age, sex (including pregnancy, childbirth, breastfeeding, or related medical conditions), gender, gender identity, gender expression, sexual orientation, veteran and/or military status (disabled veteran, veteran of the Vietnam era, other covered veteran status), political affiliation, and any other status protected by state or federal law.
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