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Northwestern University

Research Technologist 2 - Naghavi Lab

Northwestern University, Chicago, Illinois, United States, 60290


Department:

MED-Microbiology-ImmunologySalary/Grade:

NEX/11

Job Summary:Conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, and/or computer tasks and/or assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.Specific Responsibilities:Following protocols, designs, sets-up and conducts basic experiments.Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy.Using a fully functioning level of skill, completes the following activities:Molecular Biology:DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blottingPCR, RT-PCRPlasmid preparationCloning, mapping and sequencing of DNA Recombinant virus production and purificationCellular Biology:Cell culture, cell isolation Functional studies TransfectionHistology:Tissue fixation/processing: embedding, preparation of blocks and sectioning, stainingImmunohistochemistry MicroscopyImmunology:Flow cytometry ELISA, Elispot assay Cytokine assayAntibody production and purificationAnimal:HusbandryBreeding and screening transgenic mice DissectionsBlood collection Surgical proceduresDatabase management of colonyAdministration:Maintains detailed records of results which may include collecting, extracting & entering data.Manipulates, interprets &/or presents results.Prepares charts & graphs for presentations & publications.Performs scientific literature searches in support of research.Completes portions of grant applications, manuscripts & publications.Finance:Tracks & summarizes lab supply expenses.Verifies accuracy of invoices.Orders &/or maintains inventory of reagent, mice &/or lab supplies.Supervises:Trains students.Miscellaneous:Performs other duties as assigned.Minimum Qualifications:Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience.Minimum Competencies: (Skills, knowledge, and abilities.)Demonstrated laboratory experience;Demonstrated ability to follow detailed instructions as required by research protocols;Demonstrated computer skills, including spreadsheets and basic computations;Attention to detail and accurate record keeping;Ability to work independently and in a team environment;Manual dexterity.

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