The Rockefeller University
Research Assistant | Laboratory of RNA Structural Biology and Biophysics
The Rockefeller University, New York, New York, United States,
Organization Overview
The Laboratory of Structural Biology and Biophysics, led by Steve Bonilla, is dedicated to unraveling the fundamental physical principles that govern the three-dimensional shape and dynamic behavior of RNA molecules. Specifically, our focus lies in understanding the RNA sequences encoded by viruses, which enable them to manipulate the biochemical machinery of their host. To achieve this goal, we employ a wide range of cutting-edge tools and techniques, including cryogenic electron microscopy (cryo-EM), single-molecule FRET, small-angle X-ray scattering (SAXS), as well as other biophysical and structural biology methods, alongside functional assays.
Overview
The research assistant will support research conducted at the Laboratory of Structural Biology and Biophysics, assisting with molecular biology and structural characterization of RNA and RNA-protein complexes.
Responsibilities
Responsibilities **
Designing DNA templates and primers, PCR amplification, DNA purificationProducing RNA by in vitro transcription and purify by gel electrophoresisExpressing and purifying proteinsAnalyzing, presenting, and discussing dataPreparing samples for cryo-EM studiesPreparing and maintaining buffers and reagents
Qualifications
Must have previous laboratory training experience in a research labAbility to follow protocolsProficiency using basic laboratory equipment such as centrifuges, spectrophotometers, and pipettesAbility to plan and perform molecular biology experiments including primer design, PCR, gel electrophoresis, DNA extraction, cloning, and sequencingFamiliarity with protein purificationAbility to prepare and maintain reagents and solutions (e.g., buffers)Ability to clearly convey scientific concepts to colleagues and supervisors
Preferred
Previous experience working with RNA is a plusPrevious experience in a structural biology lab is a plusExperience with cryo-electron microscopy is a plusExperience expressing and purifying proteinsProgramming skills is a plusHaving independently worked on their own research project is a plus
The Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.
The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller Universitys good faith and reasonable estimate of the range of possible compensation at the time of posting.
Compensation ** * Min
USD $50,000.00/Yr.
Compensation ***** Max
USD $55,000.00/Yr.
The Laboratory of Structural Biology and Biophysics, led by Steve Bonilla, is dedicated to unraveling the fundamental physical principles that govern the three-dimensional shape and dynamic behavior of RNA molecules. Specifically, our focus lies in understanding the RNA sequences encoded by viruses, which enable them to manipulate the biochemical machinery of their host. To achieve this goal, we employ a wide range of cutting-edge tools and techniques, including cryogenic electron microscopy (cryo-EM), single-molecule FRET, small-angle X-ray scattering (SAXS), as well as other biophysical and structural biology methods, alongside functional assays.
Overview
The research assistant will support research conducted at the Laboratory of Structural Biology and Biophysics, assisting with molecular biology and structural characterization of RNA and RNA-protein complexes.
Responsibilities
Responsibilities **
Designing DNA templates and primers, PCR amplification, DNA purificationProducing RNA by in vitro transcription and purify by gel electrophoresisExpressing and purifying proteinsAnalyzing, presenting, and discussing dataPreparing samples for cryo-EM studiesPreparing and maintaining buffers and reagents
Qualifications
Must have previous laboratory training experience in a research labAbility to follow protocolsProficiency using basic laboratory equipment such as centrifuges, spectrophotometers, and pipettesAbility to plan and perform molecular biology experiments including primer design, PCR, gel electrophoresis, DNA extraction, cloning, and sequencingFamiliarity with protein purificationAbility to prepare and maintain reagents and solutions (e.g., buffers)Ability to clearly convey scientific concepts to colleagues and supervisors
Preferred
Previous experience working with RNA is a plusPrevious experience in a structural biology lab is a plusExperience with cryo-electron microscopy is a plusExperience expressing and purifying proteinsProgramming skills is a plusHaving independently worked on their own research project is a plus
The Rockefeller University does not discriminate in employment on the basis of race, color, religion, sex (including pregnancy, gender identity), national origin, political affiliation, sexual orientation, marital status, disability, genetic information, age, membership in an employee organization, retaliation, parental status, military service or other non-merit factor. All qualified applicants will receive consideration for employment without regard to the characteristics listed above.
The salary of the finalist selected for this role will be set based on various factors, including but not limited to organizational budgets, qualifications, experience, education, licenses, specialty, and training. The hiring range provided represents The Rockefeller Universitys good faith and reasonable estimate of the range of possible compensation at the time of posting.
Compensation ** * Min
USD $50,000.00/Yr.
Compensation ***** Max
USD $55,000.00/Yr.