Pfizer
Senior Associate Scientist, Protein Solutions
Pfizer, Bothell, Washington, United States, 98021
Why Patients Need You
Pfizer’s purpose is to deliver breakthroughs that change patients’ lives. Research and Development is at the heart of fulfilling Pfizer’s purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.
Position Summary
We are seeking a highly capable, enthusiastic and motivated research associate to assist in the expression of recombinant proteins using both molecular biology and mammalian tissue culture as part of the core protein expression and purification group. This person will play a critical role in supporting preclinical efforts in developing novel antibody-based therapeutics.
Principal Responsibilities
Expression of recombinant proteins, including antigens and antibodies via mammalian cell culture.
DNA preparation and vector assembly using standard molecular biology techniques.
Documentation and tracking of reagents and requests via ELN and LIMS system.
Data analysis, report generation and presentation of results.
Works with expression peers to fulfill protein reagent requests for Research.
General lab support as necessary (e.g., Buffer prep, Inventory resupply, etc.).
Required Qualifications
Bachelor of Science degree and 2-5 years lab experience in an academic and/or industry environment or equivalent.
Protein production via transient transfection.
Sterile technique for mammalian cell cultures.
Experienced in balancing workload and changing priorities.
Detail-oriented with strong analytical, organizational and written/oral communication skills.
Ability to perform routine tasks independently, more advanced tasks under direction and complete work as part of a team.
Preferred Qualifications
2-5 years of protein expression experience.
Transient transfection with Expi293 and/or ExpiCHO cells.
Design and implementation of media optimization experiments.
Protein expression using small volume plate based techniques.
Experience using Hamilton or Tecan robotics platform.
Protein quantitation using BLI or other methods.
Tracking inventory and/or requests through computer systems.
Basic knowledge of antibody structure and biochemistry.
Additional Information:
Work Location Assignment: On Premise.
Last Day to Apply: November 1, 2024.
The annual base salary for this position ranges from $64,600.00 to $107,600.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage.
Relocation assistance may be available based on business needs and/or eligibility.
EEO & Employment Eligibility
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer. This position requires permanent work authorization in the United States.
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Pfizer’s purpose is to deliver breakthroughs that change patients’ lives. Research and Development is at the heart of fulfilling Pfizer’s purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.
Position Summary
We are seeking a highly capable, enthusiastic and motivated research associate to assist in the expression of recombinant proteins using both molecular biology and mammalian tissue culture as part of the core protein expression and purification group. This person will play a critical role in supporting preclinical efforts in developing novel antibody-based therapeutics.
Principal Responsibilities
Expression of recombinant proteins, including antigens and antibodies via mammalian cell culture.
DNA preparation and vector assembly using standard molecular biology techniques.
Documentation and tracking of reagents and requests via ELN and LIMS system.
Data analysis, report generation and presentation of results.
Works with expression peers to fulfill protein reagent requests for Research.
General lab support as necessary (e.g., Buffer prep, Inventory resupply, etc.).
Required Qualifications
Bachelor of Science degree and 2-5 years lab experience in an academic and/or industry environment or equivalent.
Protein production via transient transfection.
Sterile technique for mammalian cell cultures.
Experienced in balancing workload and changing priorities.
Detail-oriented with strong analytical, organizational and written/oral communication skills.
Ability to perform routine tasks independently, more advanced tasks under direction and complete work as part of a team.
Preferred Qualifications
2-5 years of protein expression experience.
Transient transfection with Expi293 and/or ExpiCHO cells.
Design and implementation of media optimization experiments.
Protein expression using small volume plate based techniques.
Experience using Hamilton or Tecan robotics platform.
Protein quantitation using BLI or other methods.
Tracking inventory and/or requests through computer systems.
Basic knowledge of antibody structure and biochemistry.
Additional Information:
Work Location Assignment: On Premise.
Last Day to Apply: November 1, 2024.
The annual base salary for this position ranges from $64,600.00 to $107,600.00. In addition, this position is eligible for participation in Pfizer’s Global Performance Plan with a bonus target of 7.5% of the base salary. We offer comprehensive and generous benefits and programs to help our colleagues lead healthy lives and to support each of life’s moments. Benefits offered include a 401(k) plan with Pfizer Matching Contributions and an additional Pfizer Retirement Savings Contribution, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage.
Relocation assistance may be available based on business needs and/or eligibility.
EEO & Employment Eligibility
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer. This position requires permanent work authorization in the United States.
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