RA II/Sr. RA Downstream Production & Process Development

615942

Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.

Required Skills:

• BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
• 2+ years of academic or biotech/pharma laboratory experience.
• Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.
• Experience with protein refolding from inclusion bodies and/or protein modification is a plus.

Responsibilities:

• Optimize protein refolding conditions from inclusion bodies.
• Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
• Execute enzymatic and chemical protein modification protocols.
• Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.
• Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.
• Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.
• Present data routinely to the Protein Sciences group and other multidisciplinary groups.

Compensation within this range will be commensurate with level of experience