Planet Pharma
RA II/Sr. RA Downstream Production & Process Development
Planet Pharma, Cambridge, Massachusetts, us, 02138
615942
Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.
Required Skills:BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.2+ years of academic or biotech/pharma laboratory experience.Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.Experience with protein refolding from inclusion bodies and/or protein modification is a plus.
Responsibilities:Optimize protein refolding conditions from inclusion bodies.Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.Execute enzymatic and chemical protein modification protocols.Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.Present data routinely to the Protein Sciences group and other multidisciplinary groups.
Compensation within this range will be commensurate with level of experience
Our client, a leading Biotechnology company in Cambridge. MA, is looking for a Research Associate II/Sr. RA or Downstream Production & Process Development to work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC.
Required Skills:BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.2+ years of academic or biotech/pharma laboratory experience.Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.Experience with protein refolding from inclusion bodies and/or protein modification is a plus.
Responsibilities:Optimize protein refolding conditions from inclusion bodies.Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.Execute enzymatic and chemical protein modification protocols.Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.Present data routinely to the Protein Sciences group and other multidisciplinary groups.
Compensation within this range will be commensurate with level of experience