Vertex Pharmaceuticals
Protein Biochemist - Research Scientist/Senior Research Scientist (Contract)
Vertex Pharmaceuticals, San Diego, California, United States, 92189
Job Description:Contractor Protein Sciences - Research Scientist/Senior Research ScientistVertex Pharmaceuticals is seeking a highly motivated scientist with expertise in Protein Expression and Purification, and a track record of generating high-quality recombinant protein for biochemistry, biophysics and structural biology experiments.Key Responsibilities:
Expression, purification, and characterization of mammalian integral membrane proteins for assay development and biophysical experiments.Design and implement protein purification protocols to generate high quality, homogeneous protein, using various chromatography methods such as affinity, size exclusion, ion exchange.Proactively troubleshoot the expression and purification of challenging targets to ensure consistent deliveries of high-quality protein.Characterization of the stability and homogeneity of purified proteins using techniques such as FPLC, HPLC, SDS-PAGE, LC-MS.An open mindset and willingness to learn new techniques as needed to support the Protein Sciences group is desirable.Requirements
Minimum requirement is Bachelor's degree and 2 years' experience in a research laboratory. Post-graduate degree in Biochemistry, Molecular Biology, or a related field, and/or industry experience is a plus.Proven record and expertise in molecular biology and protein chemistry techniques. Experience in membrane protein purification, and CryoEM sample preparation is a plus.Team oriented, with the ability to work effectively in a fast-paced, timeline-driven work environment.Excellent organizational and documentational practices.Pay Range:
$50 - $60/HR
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Expression, purification, and characterization of mammalian integral membrane proteins for assay development and biophysical experiments.Design and implement protein purification protocols to generate high quality, homogeneous protein, using various chromatography methods such as affinity, size exclusion, ion exchange.Proactively troubleshoot the expression and purification of challenging targets to ensure consistent deliveries of high-quality protein.Characterization of the stability and homogeneity of purified proteins using techniques such as FPLC, HPLC, SDS-PAGE, LC-MS.An open mindset and willingness to learn new techniques as needed to support the Protein Sciences group is desirable.Requirements
Minimum requirement is Bachelor's degree and 2 years' experience in a research laboratory. Post-graduate degree in Biochemistry, Molecular Biology, or a related field, and/or industry experience is a plus.Proven record and expertise in molecular biology and protein chemistry techniques. Experience in membrane protein purification, and CryoEM sample preparation is a plus.Team oriented, with the ability to work effectively in a fast-paced, timeline-driven work environment.Excellent organizational and documentational practices.Pay Range:
$50 - $60/HR
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